ILPS Standardized Methods for Lecithin Analysis

The global fats and oils industry relies heavily on the analytical integrity of the Official Methods and Recommended Practices of the American Oil Chemists' Society. The Methods are critical to processing, trading, utilizing, and evaluating fats, oils, and lipid products. Over 400 analytical methods, tried and tested by collaborative studies, with corroborated results and proven global reliability, are included. AOCS' Methods reflect ongoing cooperative efforts to achieve global harmonization of analytical methodology with organizations such as the Federation of Oils, Seeds and Fats Associations (FOSFA) International, the International Standards Organization (ISO), and the International Union of Pure and Applied Chemistry (IUPAC). The AOCS has adopted the ISO Standard 5725-1986 for statistical analysis and the AOAC-IUPAC-ISO protocols for collaboratives.

Product Method List by Section

(From AOCS Official Methods)

Moisture

Ja 2a-46 (97)

Moisture, Karl Fischer Reagent

Ja 2b-87 (97)

Hexane-Insoluble Matter

Ja 3-87 (97)

Acetone-Insoluble Matter

Ja 4-46 (00)

Phosphorus, Total

Ja 5-55 (89)

Acid Value

Ja 6-55 (97)

Phospholipids in Lecithin Concentrates by TLC

Ja 7-86 (97)

Phospholipids in Lecithin Concentrates by HPLC

Ja 7b-91 (97)

Peroxide Value

Ja 8-87 (97)

Gardner Color

Ja 9-87 (01)

Brookfield Viscosity

Ja 10-87 (97)

Viscosity of Transparent Liquids by Bubble Time Method

Ja 11-87 (97)

Analysis of Lecithin Co-Products

Ja 12-89 (97)

Determination of Tocopherols in Lecithin by HPLC

Ja 13-91 (97)

Determination of Iodine Value in Lecithin, Wijs Method

Ja 14-91 (97)

Methods

Methods is an important guide and reference for every edible products analytical laboratory. Definition and scope are stated for each method, as well as details for apparatus, reagents, and procedures to be used. Relevant calculations and notes are provided to guide the technician through each procedure. Methods is published as a 1,200-page, one-volume edition, with convenient loose-leaf pages, bound by practical, heavy-duty binders.

To order, contact the AOCS technical department via e-mail at technical@aocs.org or fax your request to AOCS at 1-217-351-8091. You may also call AOCS at 1-217-359-2344 or write to us at: AOCS, P.O. Box 3489, Champaign, IL 61826-3489, USA. The cost of the Official Methods and Recommended Practices of the American Oil Chemists' Society is $500 each, plus U.S. $20 postage and handling within the U.S., U.S. $95 outside the U.S. Only IL residents should add 7.25% sales tax.

In Europe approved DGF official methods are also used. There may be slight differences in the procedures, but a number of methods is identical. For scientific and commercial purposes the used method should be referred. Below you will find the list with comparable methods.

 

DGF

AOCS

EU Regulation

Acetone Insoluble

F-I 5 (98)

Ja 4-46 (00)

 

Toluene Insoluble / Hexane Insoluble

F-I 4b (

Ja 3-87 (97)

EU 81/712, method 10

Moisture KF

F-I 4 (02)

Ja 2b-87 (97)

 

Acid Value

F-I 3 (00)

Ja 6-55 (97)

 

Peroxide Value

F-I 3b (00)

Ja 8-87 (97)

EU 81/712, method 9

Gardner Color

C-IV 4a (02)

Ja 9-87 (01)

 

Viscosity

F-I 2 (00)

Ja 10-87 (97)

 

Phosphorus

F-I 5a (00)

Ja 5-55 (89)

 

Iron

F-I 4a (98)

Ca 15-75 (97)

 

Iodine Value (Wijs)

F-I 3a (02)

Ja 14-91 (97)

 

HPTLC

F-I 6 (99)

 

 

HPLC (LSc)

F-I 6a (00)

Ja 7c-07 (07)

 

Sampling

F-I 1 (68)

Ja 12-89 (97)

 

w-3 FA Analysis

1H- and 13C NMR spectra are sensitive for chemical structures, different subgroups of similar chemical surroundings are separated in regions of similar chemical shifts.

Any common fatty acid consists of a terminal methyl group. Saturated and unsaturated fatty acids show different chemical shifts of these methyl groups. The difference in the chemical shift depends on the distance between the terminal methyl group and the position of the next double bond.

If you are interested in the w-3 FA Analysis method, feel free to download the following doument:

Determination of w-3 fatty acids by 1H and 13C NMR spectroscopy